Transcript Poland Workshop - Western Illinois University

International Hands-on workshop on
genotoxiciy monitoring bioassays for
environmental pollution
University of Silesia, Jagiellonska 28,
40-032 Katowice, Poland
Directed by
Prof. Jolanta Maluszynska, Department of Plant Anatomy and
Cytology, University of Silesia, Katowice, Poland
Prof. Siegfried Knasmueller, Institute for Cancer Research, The
Medical University of Vienna, Vienna, Austria
Prof. Michael Plewa, University of Illinois, Champaign-Urbana,
IL, USA
International workshop on genotoxicity monitoring
bioassays for environmental pollution
Group photo of the participants
Prof. Knasmueller (left) and Prof. Maluszynska (right) discuss workshop plan
Prof. Jolanta Maluszynska giving instructions at the workshop,
demonstrating Fluorescent in situ hybridization (FISH) assay
(photomicrograph was not available)
Instructor of the workshop giving a lecture to the participants.
Prof. Michael Plewa of the University of Illinois demonstrating the slide
preparation for Comet test for clastogens
Photomicrograph of a comet tail as the result of DNA
damage by clastogens
Mr. Bernhard Majer of the medical University of Vienna prepares Allium roots
and Tradescantia cuttings for chromosome aberration and micronucleus assays .
Tradescantia plants bearing young inflorescences provide the test material for both
Tradescantia- Stamen-Hair (Trad-SHM) and Tradescantia-Micronucleus (Trad-MCN) assays
Photomicrograph of Sister-ChromatidPhotomicrograph of Sister-ChromatidExchange (SCE) in Tradescantia root tip cell
Exchange (SCE) in Tradescantia root tip cell
cell.
Dissection of flower buds in preparation of Trad-MCN slides from the
meiotic microsporocytes
Preparing stamen hairs of Tradescantia flower under a dissecting microscope for scoring
frequencies of pink mutation events in the Trad-SHM assay.
MCN
MCN
Photomicrograph showing micronuclei (MCN) among the resting
stage root tip cells in the subsequent generation of treated nuclei
MCN
MCN
MCN
Photomicrograph of the tetrad stage of microsporocyte of Tradescantia showing
micronuclei (MCN) among the synchronized tetrads in Trad-MCN assay
Scoring pink mutation events under the dissecting microscope in the Tad-SHM assay
Tradescantia flowers ready for scoring pink mutation events after a 16- minute refrigeration
in the Trad-SHM assay
blue
pink
pink
blue
Photomicrograph of orderly aligned stamen hairs showing 3 pink mutation
events in this field of view in the Trad-SHM assay
Collecting data for frequencies of mutation and micronueli in treated
and control groups of Trad-SHM and Trad-MCN assays
DATA analysis using computer software and print out for report write up
After a day’s hard work, participants took a tour around town
elaxing and shopping in the Mall in the city of Katowice, Poland
Let’s have some coffee and snack
A walk outside to have some fresh air