Transcript Relationship between radiation dose and chromosome

Piotr Knapp
Piotr Wróblewski
Supervisors: Ekaterina Zaytseva, Polina Kutsalo,
Marta Deperas-Kamińska
Laboratory of Radiation Biology
The fate of irradiated cell
Gy
After high dose
Correct repair
Necrotic
death
Healthy cell
Incorrect repair
No
effect
Somatic
effect
Cell with damaged DNA
Unstable
chromosomal
aberrations
Micronuclei
Mitotic death
Somatic
effect
Stable
chromosomal
Aberrations
- Mutations
Mutation path
Cancer
Carcinogenic
effect
Apoptotic death
No effect
In the
germ cells
Genetic
effect
Somatic
effect
 Creation of dose dependency calibration curve of
chromosomal aberrations in human peripherial blood
lymphocytes exposed to protons beam
(LET≤100keV/µm) performed at Phasotrone (LNP,
JINR)
 Biological dosimetry - Allow to assess an accidental
radiation dose on base of the level of changes in
chromosome structure.
Culture of
irradiated
blood
BrdU
Colcemid
Fixation
0-24h
48h
Watching
48-50h
An example of cell stained with Giemsa
dye with dicentric chromosome.
Aberration
per cell
DOSE
[Gy]
 Marta Deperas-Kamińska
 Polina Kutsalo
 Ekaterina Zaytseva