Transcript Slide Show from 2013 Class

Jim Thompson
Ron Woodruff
Clay Hallman
These creatures
are hard to
photograph! (Jim
holds the camera,
and Clay is adept
at avoiding it.)
Jim is a David Ross Boyd Professor of
Biology at OU, and Clay is a Research
Associate.
Jim and Ron have taught this course
for about 24 years, and this is Clay’s
10th year as the Teaching Assistant.
Ron is a
Distinguished
Research
Professor at
Bowling Green
State University
in Ohio.
(Ron is simply able
to focus on other
things and, thus,
ignore the camera.)
The University of
Oklahoma Biological
Station is located on the
quiet shore of Lake
Texoma, at one time the
largest man-made reservoir
in the world.
But then, at
other times,
it was not
quite so
quiet!
We worked in two adjacent
rooms on the first floor of the
classroom building.
The Station has
wonderful resources
for small, intensive
classes like ours, as
well as support for
year-round graduate
student and faculty
researchers.
Our activities were split
between a lecture/writing
room and a separate lab,
where chemicals and other
potential hazards could be
safety used and monitored.
They also have good food!
That is good,
because there is
not much else
around for
dozens of miles.
Molecular Techniques for Field Biology
Brad Snider
Danielle Vinnedge
Rachel Flanagan
Robert Byrd
Danielle Strawn
Jennie Lee
Molecular Techniques for Field Biology
Taylor Paziuk
Adam Richardson
Barun Bouvia
Zain Hyder
James Pembrook
John Logan
Although we had four “Lab Groups”,
in practice everyone worked together.
Green:
Red:
Robert Byrd
Brad Snider
Danielle
Vinnedge
Rachel
Flanagan
John Logan
James Pembrook
Brown:
Blue:
Barun Bouvia
Zain Hyder
Danielle Strawn
Jennie Lee
Taylor Paziuk
Adam
Richardson
FRONT
Barun Bouvia
Zain Hyder
Danielle Strawn
Robert Byrd
Brad Snider
Danielle Vinnedge
Rachel Flanagan
John Logan
James Pembrook
Jennie Lee
Taylor Paziuk
Adam Richardson
Who would have
thought spots
could be so much
fun?
Seining will
probably
not become
a popular
spectator
sport.
Is it still called “field” work when it is wet?
Such a big lake
… such little
nets.
Eventually, we caught one minnow.
(We believe in quality, not quantity
– although quantity would not have
been turned away)!
The whole class prepared successful protein electrophoresis plates
from different parts of this now-famous minnow.
It’s all in the wrists!
A nice combination of
individual and team work.
Back safely from another field trip – (to the lab down the road)
At the Station we
are surrounded by
biology.
10000
8000
6000
5000
4000
3000
2500
2000
23130
9416
6557
4361
2322
2027
1500
1000
564
500
Our data were
put into the
appropriate
theoretical
framework.
(Our jokes,
however, were in
a class of their
own.)
Preparing for a guest lecture by
Dr. Philip Morton.
He engaged us
with a research
project that
involved us in
generating
hypotheses
and applying
our new
knowledge.
Using molecular genetic
databases … there is a world of
data out there!
Results from our BarCoding of tissue samples by PCR
Sample 5
>MT1-5_HCO
TATCTTGGTATAAATAGGGTCTCCTCCTCCAGCAGGATCAAAGAATGATGTATTAATATTTCGGTCTGTTAAGAGTATTGTGATTGCTCCGGCGAGTACGGGTAGAGAAA
GAAGTAATAATAAGGCAGTTAAAGCTACTGATCAGACAAATAGAGGCATTCGATCAAAAGTTATTCCTGTAGAACGCATATTAATAACAGTTGTAATAAAATTTACAGCCC
CGAGGATTGATGAGATTCCTGCAAGGTGAAGCCTAAAAATAGCTAAATCTACAGAAGCCCCTCTATGAGCGATATTAGAGGATAATGGCGGGTAAACAGTTCAACCTGT
CCCGGCCCCTCTTTCTACAAGGCTTCTCATTAAAAGAAGAGTTAGGGAAGGGGGTAGTAATCAAAATCTTATATTATTTATTCGAGGGAATGCTATATCAGGGGCACCCA
ATATAAGTGGTACAAGCCAATTTCCAAACCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCATGAGCAGTTACAATAACATTATAAATTTGGTCGTCT
CCGATTAATGTTCCCGGATTTCCAAGTTCAGCTCGAATAAGTAATCTTAATGAGGTTCCAACTATACCTGCTCATCTTCCAAAGAGAAAGTATAAGGTTCCAATATCTTTAT
BLAST result: Phyllophaga crassissima
Cytochrome Oxidase I (COI) Sequences
BLAST result: Coccinella
septempunctata
BLAST result: Chironomus
entis
BLAST result: Drosophila suzukii
BLAST result: Lucilia coeruleiviridis
BLAST result: Leptoglossus occidentalis
BLAST result: Melanoplus
BLAST results: Solenopsis
invicta
BLAST result: Larinioides cornutus
BLAST result: Solenopsis invicta
BLAST result: Estigmene
acrea
BLAST result: Phidippus audax
Schedule of Talks
Thursday afternoon, 23 May 2013
Adam Richardson
Noel, S., M. Ouellet, P. Galois, and F.-J. Lapointe. 2007.
Impact of urban fragmentation on the genetic structure of the eastern red-backed salamander.
Conserv. Genet. 8: 599-606.
Robert Byrd
Marshall, J.C., Jr., B.A. Kingsbury, and D.J. Minchella.
2009. Microsatellite variation, population structure, and bottlenecks in the threatened
copperbelly water snake. Conserv. Genet. 10: 465-476.
John Logan
Miller, C.R., and L.P. Waits. 2003. The history of
effective population size and genetic diversity in the Yellowstone grizzly (Ursus arctos):
Implications for conservation. Proc. Natl. Acad. Sci. USA 100(7): 4334-4339.
Taylor Paziuk
Omorato, D.P., E.C. Hellgren, R.A. Van Den Bussche, D.L.
Doan-Crider, and J.R. Skiles, Jr. 2007. Genetic structure of American black bears in the desert
southwest of North America: conservation implications for recolonization. Conserv. Genet. 8:
565-576.
Brad Snider
Dixon, J.D., M.K. Oli, M.C. Wooten, T.H. Eason, J.W.
McCown, and M.W. Cunningham. 2007. Genetic consequences of habitat fragmentation and
loss: the case of the Florida black bear (Ursus americanus floridanus). Conserv. Genet. 8: 455464.
James Pembrook
Luo, S.-J., et al. 2004. Phylogeny and genetic ancestry of
tigers (Panthera tigris). PLoS Biology 2: 2275-2293.
Friday morning, 24 May 2013
Barun Bouvia
Reed, D.H., V.-H. Teoh, G.E. Stratton, and R.A. Hataway.
2009. Levels of gene flow among populations of a wolf spider in a recently fragmented habitat:
current versus historical rates. Conserv. Genet. DOI 10.1007/s10592-009-9995-9.
Jennie Lee
Spradling, T.A., J.W. Tamplin, S.S. Dow, and K.J. Meyer.
2010. Conservation genetics of a peripherally isolated population of the wood turtle (Glyptemys
insculpta) in Iowa. Conserv. Genet. DOI 10.1007/s10592-010-0059-y.
Danielle Strawn
Vonholdt, B.M., D.R. Stahler, D.W. Smith, D.A. Earl, J.P.
Pollinger, and R.K. Wayne. 2008. The genealogy and genetic variability of reintroduced
Yellowstone grey wolves. Molec. Biol. 17: 252-274.
Rachel Flanagan
Schmidt, J.I., K.J. Hundertmark, R.T. Bowyer, and K.G.
McCracken. 2009. Population structure and genetic diversity of moose in Alaska. J. Heredity
100: 170-180.
Danielle Vinnedge
Zakhov, E.V., and J.J. Hellmann. 2008. Genetic
differentiation across a latitudinal gradient in two co-occurring butterfly species: revealing
population differences in a context of climate change. Molec. Ecol. 17: 189-208.
Zain Hyder
Huchon, D., P. Chevret, U. Jordan, C.W. Kilpatrick, V.
Ranwez, P.D. Jenkins, J. Brosius, and J. Schmitz. 2007. Multiple molecular evidences for a
living mammalian fossil. Proc. Natl. Acad. Sci. USA 104(18): 7495-7499.
preparing a research talk on a
paper using various molecular
techniques – our “final exam”
Molecular Techniques for Field Biology – 2013