Transcript بسم الله الرحمن الرحيم GROWTH AND NUTRITION OF BACTERIA
ميحرلا نمحرلا الله مسب
GROWTH AND NUTRITION OF BACTERIA
Prof. Khalifa Sifaw Ghenghesh
Dept. of Medical Microbiology, Faculty of Medicine, Tripoli University Tripoli - Libya
Growth:
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An Orderly Increase in All components of an Organism Multiplication >> Binary Fission.
Measurement of Growth:
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Increase in Numbers Increase in Mass
Batch Culture:
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Growth Curve:
A Total Count.
A Viable Count.
1.
Lag Phase 2.
Log (logarithmic) Phase 3.
Stationary Phase 4.
Death Phase
GROUTH REQUIREMENTS AND BACTERIAL NUTRITION:
Suitable Nutrients Must Be Supplied Physical Conditions Must Be as Near as Possible for the Organism Under Consideration
BACTERIAL METABOLISM: Bacteria Require: An Energy Source A Carbon Source A Nitrogen Source Other Inorganic Salts and Accessory Growth Factors.
1.
Photo-autotrophs: 2.
Chemo- autotrophs 3.
Heterotrophs:
OXYGEN REQUIREMENTS AND METABOLISM: 1.
Obligate Aerobes 2.
3.
Obligate Ananerobes Facultative Anaerobes 4.
Microaerophilic Bacteria.
MEDIA FOR BACTERIAL GROWTH: 1.
2.
3.
General Laboratory Media:
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Peptone >> Amino acids Growth factors and inoorganic salts NaCl Synthetic Media Special Media:
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Selective media
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Indicator media Enrichment media
PHYSICAL CONDITIONS REQUIRED FOR GROWTH 1.
Influence of Oxygen and CO: 2.
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Influence Temperature: On Growth:
Mesophilic bacteria Psychrophilic bacteria Thermophilic bacteria 3.
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On Viability: Influence of Moisture and Desiccation:
4. Influence of Hydrogen Ion Concentration (pH): 5. Influence of Light and other radiations: 6. Influence of Osmotic Pressure: 7. Influence of Mechanical and Sonic Stresses: