Transcript Making TEM specimen

EM Course – Making of Thin Films
Professor Rodney Herring
Making STEM Specimen
We will look at each one of these methods in turn.
Making STEM Specimen – Dispersion Method
Making STEM Specimen – Microtomy Method
Making S(T)EM Specimen – Focus Ion Beam (FIB),
Ion Mill, Plasma/UV Cleaner Method
Making STEM Specimen – Focus Ion Beam (FIB)
Method
Making STEM Specimen – Focus Ion Beam (FIB)
Method
Making STEM Specimen – Focus Ion Beam (FIB)
Method
Making a Thin Cross-sectional Specimen by FIB
The FIB can weld any two
pieces of material together
at the nanoscale
Welding specimen to 33mm Cu grid
Limited Micro/Nano Machining
Lab on a chip
MEMS
Viewing of Cell Cluster
Cell Tissue (No Fixation)
Tobacco flower petals
Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK
Cell Tissue (No Fixation)
Tobacco flower petals
Metal
deposition
Samples: Tobacco Flower Petals
Courtesy: Kim Findlay, John
Innes Centre, Norwich, UK
Cell Tissue (No Fixation)
Tobacco flower petals
Samples: Tobacco Flower Petals
Courtesy: Kim Findlay, John
Innes Centre, Norwich, UK
Features never before seen were found in these specimens.
Making STEM Specimen – Focus Ion Beam (FIB)
Method