Transcript I. Zinicovscaia
Department of Neutron Activation Analysis Division of Nuclear Physics Frank Laboratory of Neutron Physics Joint Institute for Nuclear Research
Biosynthesis of silver and gold nanoparticles using microbial biomass
Inga Zinicovscaia
E-mail: [email protected]
JINR Scientific Council, September 15-16, 2011
M.V. Frontasyeva, S.S. Pavlov
Frank Laboratory of Neutron Physics , JINR, Russian Federation
T.
Kalabegishvili , E. Kirkesali , I. Murusidze , D. Pataraya, E.N. Ginturi
Andronikashvili Institute of Physics, Tbilisi , Georgia
I. Zinicovscaia, Gh. Duca
Institute of Chemistry of the Academy of Science of Moldova, Chisinau, Moldova
Introduction
An important area of research in nanotechnology deals with
synthesis of nanoparticles
of different chemical composition and size. There is a growing need to develop
nanoparticle synthesis
chemicals in its process.
environmentally gentle
that does not use toxic As a result, researchers in the field of nanoparticle synthesis have turned to biological systems. It is well known that many organisms, both uni-cellular and multi-cellular, are producing inorganic materials either intra- or extra-cellularly.
3
Advantages of biological method
tightly controlled, highly reproducible syntheses
biocompatible particles
the avoidance of toxic surfactants or organic solvents
4
Biotechnology of silver nanoparticles
Application of silver nanoparticles
nonlinear optics medicine electronics catalysis microelectronics
6
Experiment +
AgNO
3 1
mM =
pale yellow yellowish brown UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM silver nitrate and 1 g Streptomyces glaucus 71MD
7
Scanning electron microscope
Resolution 1.2 nm Magnification 5000–150000x Voltage 1–30 kV
Quanta 3D FEG
The Netherlands’ Firm “Systems for Microscopy and Analysis” (Moscow, Russia) 8
Objects of study
p
Blue-green
microalga
Spirulina platensis
Bacteria
Streptomyces glaucus
71MD 9
Control 1 day SEM micrographs of Spirulina platensis cells with silver nanoparticles
10
C O
EDAX spectrum recorded from Spirulina platensis cells after formation of silver nanoparticles
11
150 000x 50 000x Control SEM micrographs of Streptomyces glaucus 71MD cells with silver nanoparticles
12
EDAX spectrum of Streptomyces glaucus 71MD cells after exposure to silver nitrate solution
13
Biotechnology of gold nanoparticles
Application of gold
nanoparticles
Catalysis
Chemical sensing
Biosensing
Medicine
15
Experiment +
HAuCl
4 = yellow red purple
Experiment I
C HAuCl4
= 1mM Incubation time: 1 − 6d
Experiment II
Incubation time = constant
C HAuCl4 :
10 -4 − 10 -2 M 16
UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM hydrated gold chloride and 1 g biomass of A. globiformis 151B
17
Experiment I 5 days 2 days Control SEM micrographs of Spirulina platensis cells with gold nanoparticles at different incubation time
18
Experiment II HAuCl 4 10 -4 -3 M 10 -2 M M SEM micrographs of Spirulina platensis cells with gold nanoparticles at different concentrations
19
EDAX spectrum of Sp. platensis cells after exposure to hydrated gold chloride solution
20
Conclusions
1 .
Production of silver and gold nanoparticles by blue-green microalgae bacteria
Spirulina platensis Streptomyces glaucus
and 71MD proceeds extra-cellularly 2. SEM and EDAX were used to characterize the silver and gold nanoparticles. SEM showed formation of nanoparticles in the range of: a) 4–25 nm for
Streptomyces glaucus
71MD b) 16–200 nm for experiment) c) 15 nm–7 μm for experiment)
Spirulina platensis Spirulina platensis
(first (second 21
For quantitative analysis of samples the epithermal neutron activation analysis (ENAA) in the radioanalytical complex REGATA at the reactor IBR-2M will be carried out by the end of 2011 22
Elemental concentration in biomass of Streptomyces glaucus (irradiation time 8 s) Element Energy, keV Concentration, µg/g Error, % Ag K La Mn Na Sb
657.76
1524.58
1596.21
846.75
1368.55
564.24
37 3290 15 25.0
381 1.3
5 8 14 6 5 15
The data were obtained by Sergey Pavlov (FLNP JINR) and Arnaud Faanhof (NECSA) in June 2011 at the reactor SAFARI-I 23