I. Zinicovscaia

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Transcript I. Zinicovscaia

Department of Neutron Activation Analysis Division of Nuclear Physics Frank Laboratory of Neutron Physics Joint Institute for Nuclear Research

Biosynthesis of silver and gold nanoparticles using microbial biomass

Inga Zinicovscaia

E-mail: [email protected]

JINR Scientific Council, September 15-16, 2011

M.V. Frontasyeva, S.S. Pavlov

Frank Laboratory of Neutron Physics , JINR, Russian Federation

T.

Kalabegishvili , E. Kirkesali , I. Murusidze , D. Pataraya, E.N. Ginturi

Andronikashvili Institute of Physics, Tbilisi , Georgia

I. Zinicovscaia, Gh. Duca

Institute of Chemistry of the Academy of Science of Moldova, Chisinau, Moldova

Introduction

An important area of research in nanotechnology deals with

synthesis of nanoparticles

of different chemical composition and size. There is a growing need to develop

nanoparticle synthesis

chemicals in its process.

environmentally gentle

that does not use toxic As a result, researchers in the field of nanoparticle synthesis have turned to biological systems. It is well known that many organisms, both uni-cellular and multi-cellular, are producing inorganic materials either intra- or extra-cellularly.

3

Advantages of biological method

tightly controlled, highly reproducible syntheses

biocompatible particles

the avoidance of toxic surfactants or organic solvents

4

Biotechnology of silver nanoparticles

Application of silver nanoparticles

    

nonlinear optics medicine electronics catalysis microelectronics

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Experiment +

AgNO

3 1

mM =

pale yellow yellowish brown UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM silver nitrate and 1 g Streptomyces glaucus 71MD

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Scanning electron microscope

Resolution 1.2 nm Magnification 5000–150000x Voltage 1–30 kV

Quanta 3D FEG

The Netherlands’ Firm “Systems for Microscopy and Analysis” (Moscow, Russia) 8

Objects of study

p

Blue-green

microalga

Spirulina platensis

Bacteria

Streptomyces glaucus

71MD 9

Control 1 day SEM micrographs of Spirulina platensis cells with silver nanoparticles

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C O

EDAX spectrum recorded from Spirulina platensis cells after formation of silver nanoparticles

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150 000x 50 000x Control SEM micrographs of Streptomyces glaucus 71MD cells with silver nanoparticles

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EDAX spectrum of Streptomyces glaucus 71MD cells after exposure to silver nitrate solution

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Biotechnology of gold nanoparticles

Application of gold

nanoparticles 

Catalysis

Chemical sensing

Biosensing

Medicine

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Experiment +

HAuCl

4 = yellow red purple

Experiment I

C HAuCl4

= 1mM Incubation time: 1 − 6d

Experiment II

Incubation time = constant

C HAuCl4 :

10 -4 − 10 -2 M 16

UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM hydrated gold chloride and 1 g biomass of A. globiformis 151B

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Experiment I 5 days 2 days Control SEM micrographs of Spirulina platensis cells with gold nanoparticles at different incubation time

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Experiment II HAuCl 4 10 -4 -3 M 10 -2 M M SEM micrographs of Spirulina platensis cells with gold nanoparticles at different concentrations

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EDAX spectrum of Sp. platensis cells after exposure to hydrated gold chloride solution

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Conclusions

1 .

Production of silver and gold nanoparticles by blue-green microalgae bacteria

Spirulina platensis Streptomyces glaucus

and 71MD proceeds extra-cellularly 2. SEM and EDAX were used to characterize the silver and gold nanoparticles. SEM showed formation of nanoparticles in the range of: a) 4–25 nm for

Streptomyces glaucus

71MD b) 16–200 nm for experiment) c) 15 nm–7 μm for experiment)

Spirulina platensis Spirulina platensis

(first (second 21

For quantitative analysis of samples the epithermal neutron activation analysis (ENAA) in the radioanalytical complex REGATA at the reactor IBR-2M will be carried out by the end of 2011 22

Elemental concentration in biomass of Streptomyces glaucus (irradiation time 8 s) Element Energy, keV Concentration, µg/g Error, % Ag K La Mn Na Sb

657.76

1524.58

1596.21

846.75

1368.55

564.24

37 3290 15 25.0

381 1.3

5 8 14 6 5 15

The data were obtained by Sergey Pavlov (FLNP JINR) and Arnaud Faanhof (NECSA) in June 2011 at the reactor SAFARI-I 23

Thank you for attention!