Transcript Lysbilde 1
Industriell bruk og nytte av enzymer
by Kjartan Sandnes, Dr.
Daglig leder og forskningsleder
Biorefining salmon co-products
Enzymatic hydrolysis Water and enzymes Exact time and temp Oil Water soluble protein Sediment
Some key figures
• • • • • Norwegian sea areas 7 times the size of land area All included, Norway is the 13th largest country in the world Second largest seafood export nation 53 bill. NOK 33 mill seafood meals on the table every day
Norway 2012: «Non-edible fish raw materials»
White fish (cod, haddock, saithe) Herring Aquaculture (salmon, rainbow trout) Total co-products «Industrial fish» (to the fish meal- and oil industry 2012)
T O T A L (not for direct human consumption)
325.000
239.000
350.000
914.000
202.000
1.116.000
NOTE! Seaweeds and shellfish not included
Source: fhf, Sildesalgslaget
Source: fhf, Sintef, Sildesalgslaget
Biomega AS – a blue biorefinery
• • • • • •
2000: Marine Bioproducts AS established 2002: Production established with patented continuous enzymatic hydrolysis process. No chemical processing aids required 2007: New site acquired for new factory 2011: Six patent families filed so far 2011: 30.000 tons raw materials processed . Turnover 107 mill NOK 2012: New factory with a capacity of 60.000 tons annually (130 mill NOK inv.) - opened May 2012
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Constant efforts to improve quality (cooling systems) and logistics Raw material transportation to Biomega – heads and backbones
Page
8 Raw material transportation to Biomega – viscera in bulk by truck
New processing plant
production from May 2012
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Overview of new plant
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Hydrolysate (soluble protein)
Biorefining - current and next generation products
MB Process
Oil (fat) Sediment (( unsoluble protein) Bones (minerals, protein)
N E W A N D I N N O V A T I V E P R O C E S S E S
enzyme technology – extraction – separation - isolation
BIOACTIVE PEPTIDES MODIFIED LIPIDS PEPTONES PHOSPHOSLIPIDS FLAVOURS POWDERS EXTRACTS
E X A M P L E S
MARINE MINERALS GLUCOSAMINES CHONDROITIN BIOACTIVE PEPTIDES
How enzymes can be used
Herring Hydrolysates HH 1 HH 2 Salmon Hydrolysates MB HR 1 MB HR 2 MB HR 3 Test sample Peptide size distribution (% of total detected) > 30 000 daltons 30 000 – 20 000 daltons 20 000 – 10 000 daltons 10 000 – 5 000 daltons 5 000 - 1 000 daltons 1 000 100 daltons < 100 daltons Rough estimated average Mw 0 1.3
1.2
12.4
14.6
53.1
17.4
2100 0 0 0.2
5.2
10 66.1
18.4
1000 2.5
0.7
12.6
17.8
13.3
38.3
14.7
4900 0.2
0.2
7.1
15.5
9.7
40.5
26.7
2800 0.0
0.0
4.6
10.0
10.2
33.9
41.3
1900
SEM Carbon source Glucose, Glycerol
Nitrogen source
Ammonium salts, Nitrates,
Peptone (amino acids/peptides
) Micronutrients Phosphates, Sulphates, Trace metals, Vitamins Typical growth medium
Fungi Yeasts Bacteria Mammalian cells
Antibiotics Enzymes Vaccines Other
Saccharomyces cerevisiae Growth Curves
3 6 5 4 SEM LACH SOYP OXYE CASA GELP BDYE MBPT “good for yeasts” from supplier 2 2 highest AN from supplier 1 1 0 0 1 2 3 4 5 6 7 8 highest AN from supplier 2 9 10
Time (h)
11 12 13 14 15 16 17 18 19 20 Mask writer