Transcript Helping students understand cell ultrastructure with
CELS191 - Cell and Molecular Biology
Web based tools developed to help students understand cell ultra-structure Lisa Russell, Jo Forrester and Phil Bishop Department of Zoology University of Otago Frog cell at Anaphase
CELS191 - Cell and Molecular Biology
Cell ultra-structure
Develop the students capacity to infer function of different cell types based on their recognition of constituent organelles.
Images Katrin Geist and Richard Easingwood
CELS191 - Cell and Molecular Biology
Challenges facing large, first year biology classes:
Evident that students struggle to make the link between: 3-d structure of organs, tissues and cellular components from lectures 2-d images that they obtain from light and electron micrographs
CELS191 - Cell and Molecular Biology
Challenges facing large, first year biology classes:
Textbook examples of stylised ‘typical’ cells often represent an oversimplification of real life images students will view during the course.
CELS191 - Cell and Molecular Biology
Challenges facing large, first year biology classes:
Only a very limited time and resources to provide students with hands-on practical experience with microscopy limited to light microscopy Viewing electron micrographs (TEM and SEM) restricted: paper exercises or as web-based activities free online software available has limitations (Image J and Zoomify)
CELS191 - Cell and Molecular Biology
Image J Open source program Wide range of tools, including a calibration tool Limitations: not user friendly requires detailed step by step instructions to calibrate image, annotate image etc.
students must download program x=1.40, y=0.627, angle=-131.56, length = 5.30
CELS191 - Cell and Molecular Biology
Zoomify freeware package high resolution imaging Limitations of package: unable to calibrate or calculate scale unable to add annotations or a key
CELS191 - Cell and Molecular Biology
Project
Develop a web-based tool to complement guided learning exercises that investigate: how cell ultra-structure is imaged (microscopy and scale) structure and function of common organelles cell diversity and ultra-structural differences (simple versus specialised cells)
CELS191 - Cell and Molecular Biology
Project
Collaborative project: Educational Media, Higher Education Development Centre (Peter Vlugter and Ayelet Cohen) Otago Centre for Electron Microscopy (OCEM) in particular Mr Richard Easingwood and Miss Katrin Geist Funded by a CALT (Committee for the Advancement of Learning and Teaching) e-learning grant
CELS191 - Cell and Molecular Biology
o
Project
Development of a range of high resolution EM images from a range of cell types: ‘simple cells’ (plant and animal cells) and specialised cells (secretory and absorptive)
CELS191 - Cell and Molecular Biology
Ideal features of an image viewer:
Zoom and panning tool Annotation tool Interactive key Measuring tool (linear and relative area)
CELS191 - Cell and Molecular Biology
Zoom and panning tool
CELS191 - Cell and Molecular Biology
Annotation tool
Nucleus
Dark stained areas – heterochromatin light areas - euchromatin
CELS191 - Cell and Molecular Biology
Interactive key
CELS191 - Cell and Molecular Biology
Linear measuring tool
CELS191 - Cell and Molecular Biology
Measuring area
CELS191 - Cell and Molecular Biology
Grid tool
CELS191 - Cell and Molecular Biology
Application of the image viewer:
Image viewer is now used in three first year courses Biology 112 (Introduction to animal biology) Utilized in a practical laboratory Proportion of red muscle present within a cross section of eel tissue subjected to differing hormonal treatments
CELS191 - Cell and Molecular Biology
Image viewer is now freely available throughout the university as a plug-in to UniTube (file sharing system, wide range of file types including images, videos and audio files). Once uploaded the UniTube URL can be shared or embed it into Blackboard or any other website.
CELS191 - Cell and Molecular Biology
‘Build a cell’ game
Online interactive game designed to test students ability to link cell structure with function .
CELS191 - Cell and Molecular Biology
‘Build a cell’ game
Four levels - in each level they have to essentially build the specified cell from a range of cell components.
o simple plant and animal cell, specialised secretory and absorptive cells.
CELS191 - Cell and Molecular Biology
‘Build a cell’ game
To progress through each level: o correct cell components in roughly the correct frequency (1, 2-10 or extensive) o 3 attempts at each level, if incorrect they have to start the game again.
CELS191 - Cell and Molecular Biology
Ongoing developments
Image viewer Develop the annotation tool further o o allow students to annotate and save images for reviewing potential for assessment Build a cell game developing specific feedback