Why Dextranase L ‘Amano’?

Technical presentation

Apr-13

2 points to consider!

• • Powerful! (very high endo activity) -> Dextranase L ‘Amano’ works rapidly and efficiently on Dextran during the process -> Dextranase L ‘Amano’ dosage can be adjustable at the factory depending on the purpose PURE! (very high purity) -> Dextranase L ‘Amano’ helps better crystallization

How powerful??

The balance of sugar componentｓ, 10,000 ppm Dextran, after 50 o C, 2ppm DextranaseL treatment.

100 80 60 40 20 Dextran higher DP7 DP6 DP5 DP4 DP3 DP2 DP1 0 0 5 10 15 time (min) 20 25 Dextranase L ‘Amano’ •Very quick Dextran hydrolysis •DP2 and DP3 production 30

Tangible Advantages

• • • • • Better crystalized sugar Gain better yield Rapid reduction of viscosity Higher productivity in the capacity Shorter process time

Other advantages

• • • • Easier maintenance of the equipment Prevents clogging in the event of interruption of the process.

Energy saving Lower content of Dextran in waste water

What we observed?

Voices from the market… • • • • Quality improvement!

– Lower dextran content in the process -> less consumption of other chemicals – – -> lower quantity of waste water Better particle distribution (medium size) of raw sugar Better and solid crystal in raw sugar Yield improvement – Raw sugar yield increase up to 5-7% Save energy – Lower consumption of calcium carbonate – – Lower quantity of waste water Lower energy against raw sugar production / Mt Trouble shooting! (to add Dextranase just before re-start of the process) – Longer life of filter cloth – Lower sugar loss

•

Crystallization

Better crystal Irregular crystal Decrease of irregular crystals, increase of medium size crystals.

Delivery Cleaning Cutting DDS Dextranase 2ppm Ca(OH)2 2-4% less Diffusion juice Preliming Preliming juice 145→30ppm CO2 1 st Clear juice Liming heating CO2 2nd Carbon.

1 st Carbonation Filtr.

1 st Carbon.

juice Heat

Lower Turbidity, colour

Filtr.

Cake FP SO2 2 nd Clear juice

Lower turbidity, colour

21→11ppm Sulfur.

Filtr Sulfied juice Evap.

Lower turbidity, colour

Syrup 1 st Cryst.

1 st Sepr.

1 st Molas.

2 nd Cryst.

2 nd Sepr.

2 nd Molas.

3 rd Cryst.

3 Sepr.

Raw sugar 122→52ppm （

water consump. 23% lower Lower energy consump. (1770→1651 GJ

）

Lower long crystal, mid size particle 77→80%, lower colour 65→44

rd Final Molasses 957→690ppm

Sugar loss 2.79→2.43%

Lactate value Diffuser DDS 124ppm 38ppm Mingler 137ppm 239ppm

Dextranase Glucose/Lactate analyzer

Preliming ・ We assume the infection of

Leuconostoc

during the process since the lactate value is increased.

・ We are checking the relationship between the value and dextran amount

(Info) There are 2 types of infection

Bacteria and fungi • Comes normally during the storage of raw material in hot condition, which leads to lower yield of sugar.

• Normally a carbamate based microbiocide specially formulated to inhibit growth of bacteria and fungi in cane sugar mills. Sodium dithiocarbamate, Sodium diethydlthiocarbamate and alkyl dimethyl benzyl ammonium are used in some countries.

Leuconostoc Mesnteroides

• Under cold weather, Leuconostoc Mesnteroides makes dextran during the process, which causes filter problem and poor sugar quality (crystallization, fineness etc.) • An organic antimicrobial based on dual antibiotics with special ingredient effective for bacteriostatic and bactericidal action, Sodium dithiocarbamate is also used.

• Through our experience in Japan, only Dextranase is used controlling MK value during the process (no anti-biotic agents at all).

Contact us!

Amano Enzyme Europe Ltd. Swiss Branch WTC Lugano CH-6982 Agno +41 – 91- 604 6450 [email protected]