Transcript Enzymy - inhibicja

Inhibicja kompetycyjna
S
v0 
5
Vmax [S]
Km  [S]
v0 
CI
Vmax [S]
K m  [S]
EI
S+E
ES
E+P
E
+
I
1 Km 1
1
1 K m 1
1




v0 Vmax [S] Vmax
v0 Vmax [S] Vmax
2.5
No I
+C I
2
v,0 µmol/min
0.5Vm ax
+C I
3
2
Km
Kmapp
-1/Km
1
app
1/v,0 /µmol/min
/µmol/min
1/v,
4
-1/K
-1/K
m
m
Kmapp/Vmax
1.5
1
0.5
K /Vm ax
K m
/V
m m ax
1/V 1/V
m axm ax
0
0
0
10
30
20
[S], m M
40
50
-0.6 -0.4 -0.2
0 0.2 0.4 0.6 0.8
1/[S], /m M
CI – inhibitor kompetycyjny; S – substrat; E – enzym; No I – brak inhibitora
1
No I
Inhibicja niekompetycyjna
EI
S
v0 
E
Vmax [S]
Vmax [S]
v0 
Km  [S]
K m  ' [S]
NCI
S+E
+
I
S
ESI
ES
+
I
1 K m 1
'


v 0 Vmax [S] Vmax
1 Km 1
1


v0 Vmax [S] Vmax
55
E+P
2.5
No I
0.5V
33
+ NCI
0.5V
max
2
m ax
0.5Vmax
1/v,0 /µmol/min
/µmol/min
1/v,
v,0 µmol/min
44
22
11
K
-1/K
-1/K
m
m
Km
1.5
1/Vmaxapp
Km/Vmaxapp
+ NC I
1
0.5
m
K mK/Vm
/V
m ax
m ax
1/Vm ax
1/V
00
m ax
0
00
10
10
20
20
30
30
[S],
[S], m
mM
M
40
40
50
50
-0.6 -0.4 -0.2
0 0.2 0.4 0.6 0.8
1/[S], /m M
NCI – inhibitor niekompetycyjny; S – substrat; E – enzym; No I – brak inhibitora
1
No I
Inhibicja akompetycyjna
EI
S
v
0
Vmax [S]
Km  [S]
v
0
E
Vmax [S]
Km   ' [S]
UCI
S+E
ES
+
I
1 Km 1
'


v0 Vmax [S] Vmax
1 Km 1
1


v0 Vmax [S] Vmax
5
E+P
2.5
No I
4
2
m ax
+ UC I
0.5V
max
3
Km
2
/µmol/min
1/v,0 /µmol/min
v,0 µmol/min
0.5V
0.5Vmax
app
Km
1
K
-1/Km
m
-1/K
m
-1/K
app
m
1.5
1/Vmaxapp
Kmapp/Vmaxapp
Km/Vm ax
+ UC I
K /V
m m ax
No I
1
1/V
0.5
m ax
1/V
0
m ax
0
0
10
20
30
[S], m M
[S].
40
50
-0.6 -0.4 -0.2
0 0.2 0.4 0.6 0.8
1/[S]. /m M
1/[S],
UCI – inhibitor akompetycyjny; S – substrat; E – enzym; No I – brak inhibitora
1