Transcript auburn university biological safety manual

Biological Safety:
Safety for people, animals, plants
BioSafety and BioHazard Training
Office of Engineering Safety
Texas Engineering Experiment Station (TEES)
& The Dwight Look College of Engineering
“A safe, healthful, and secure environment for scholarship and research.”
Biological Safety
Key Concepts
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Hazardous Biological Substances (Biohazards)
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Bloodborne Pathogens
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Universal Precautions
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Exposure Control Plan
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Biosafety in Microbiological and Biomedical Laboratories (BMBL)
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Biological Risk Assessment
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BioSafety Level (BSL)Criteria
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BioSecurity Criteria
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Select Agents & Toxins
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BioHazard Containment, Decontamination & Disinfection
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TAMU Biological Safety Office
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Why Is Biological Safety Important?
Biological Safety
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Use of hazardous biological materials on campus including
– Biohazardous agents and bloodborne pathogens
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Bloodborne Pathogens
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Prevent disease in personnel
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Prevent release of organisms/agents into environment
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Biological Safety
What are BioHazardous Materials?
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Any biological material capable of causing harm to humans,
animals, plants
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BioHazardous Agents – Pathogens that can replicate & cause
disease include:
– Bacteria (Streptococcus pyogenes)
– Fungi (Candida, Histoplasma)
– Viruses (HIV, HBV)
– Prions (CJD)
– Parasites (Giardia, Strongyloides)
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Toxins – Microbial poisons
– Exotoxins – produced by bacteria
• Clostridium botulinum - food poisoning, one of most deadly
• Clostridium tetanii – tetanus
• Corynebacterium diphtheriae – diptheria
• Bioterrorism use
– Endotoxins – released from cell wall when bacteria disintegrates
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Biological Safety
Classification System For Biohazardous Materials
BioSafety Levels (BSL)
Risk Group 1
BSL 1
Agents not associated with disease in
healthy adult humans
Risk Group 2
BSL 2
Agents associated with human disease;
rarely serious; preventative or therapeutic
resources available
Risk Group 3
BSL 3
Agents associated with serious or lethal
human disease; preventative or therapeutic
resources available
Risk Group 4
BSL 4
Agents likely to cause serious or lethal
human disease; no preventative or
therapeutic resources available
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The Standard Code of Practice for BioSafety
Biological Safety
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Biosafety in Microbiological and Biomedical Laboratories (BMBL)
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U.S. Department of Health & Human Services
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Public Health Service
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Centers for Disease Control & Prevention & the National Institutes of Health
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http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm
Establishes criteria for:
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Biological Risk Assessment
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Principles of BioSafety
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Laboratory BioSafety Level (BSL) Criteria
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Laboratory BioSecurity Criteria
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BioHazard Containment
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Decontamination & Disinfection
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Transportation of BioHazards & Infectious Materials
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Select Agents & Toxins…
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Biological Safety
Researcher Responsibilities:
Faculty or Principle Investigator (PI)
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Files a Biological Usage Authorization (BUA)
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Trains all personnel
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Establishes emergency procedures
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Reports incidents with biologicals
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Posts biohazard warning signs
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Cooperates with Biological Safety Officer during inspection visits
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Responsibilities of TAMU PI’s
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http://researchcompliance.tamu.edu/ibc/ibcpiresp
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BioSafety Officer
TAMU Office of Research Compliance
Biological Safety
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Dr. Bruce M. Whitney
– Institutional Biological Safety Officer
– Office of Biological Safety
– Texas A&M University
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Tel:
Mailstop:
Email:
Email:
979-458-0683 or 436-1307
1112 TAMU
[email protected]
[email protected]
• http://biosafety.tamu.edu/institutional-biosafety-committee/ibcrevapp/ibcapprev
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Biological Safety
Institutional BioSafety Committee (IBC)
TAMU Office of Research Compliance
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Mandated by National Institutes of Heath (NIH)
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Must review and approve all usage of Risk Group 2 or higher
agents
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Must review and approve rDNA usage, formation of transgenic
animals, experiments involving human gene therapy
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Applies to TAMU:
– Employees & students conducting research involving infectious
biohazrds
– Research subjects, human or animal
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TAMU IBC: http://researchcompliance.tamu.edu/ibc/welcome
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http://biosafety.tamu.edu/institutional-biosafety-committee/ibcrevapp/ibcapprev
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Biological Safety
Biological Usage Authorization (BUA)
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Describes the Principle Investigator’s Research Project
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Required when biohazardous material is stored, used,
or transported
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Good for 3 years
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Can be amended
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Biological Safety
Forbidden Agents
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Cannot be brought on
campus!
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Mycobacterium tuberculosis
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Hantavirus
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Foreign Animal Pathogens
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HIV 1 & 2
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Reference material can be
kept in inactive state
(frozen or lyophilized)
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List of all references must be
given to IBC
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Biological Safety
CDC Select Agents
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Cannot be brought on campus without prior approval &
registration
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Must be registered with the University BioSafety Officer
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Required under:
• U.S. BioTerrorism Prevention Act
• USA Patriot Act (USAPA)
• TAMUS Regulations, TEES & TAMU Rules
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Biological Safety
The BioSafety Inspection!
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Personal Protective Equipment
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Training
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Signage
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Adherence to Protocol
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Technique
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Physical Facility
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General Safety
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Risk Assessment
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Controls
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Project Safety Analysis (PSA)
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Biological Safety
What You Must Learn to Work Safely with
Bloodborne Pathogens
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Cleaning
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Decontamination
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Regulated waste disposal
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Pre and post exposure procedures
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Universal Precautions (CDC)
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Special hazards
– HIV, HBV research
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Biological Safety
What are Bloodborne Pathogens?
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Microbial agents transmitted by the blood and bodily fluids of
infected individuals.
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Control of Occupational Exposures to Bloodborne Pathogens
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29 CFR 1910.1030
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http://www.osha.gov/SLTC/bloodbornepathogens/index.html
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Biological Safety
Exposure Control Plan
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All laboratory supervisors, faculty & principle investigators are
responsible for insuring their employees and students comply
with the Exposure Control Plan to reduce the risk of occupational
exposures to bloodborne pathogens.
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All personnel that have the potential to be exposed to bloodborne
pathogens MUST be trained to this plan.
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A copy of the Exposure Control Plan must be readily available in
each work area or laboratory where bloodborne pathogens
exposures exist.
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Biological Safety
Where to Get More Information
www.osha.gov/SLTC/bloodbornepathogens/index.html
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Workers in many different occupations are at risk of exposure to
bloodborne pathogens, including Hepatitis B, Hepatitis C, and
HIV/AIDS.
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In 1991, OSHA issued the Bloodborne Pathogens Standard to
protect workers from this risk.
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In 2001, in response to the Needlestick Safety and Prevention Act,
OSHA revised the Bloodborne Pathogens Standard, 29 CFR
1910.1030.
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The revised standard clarifies the need for employers to select
safer needle devices and to involve employees in identifying and
choosing these devices.
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The updated standard also requires employers to maintain a log
of injuries from contaminated sharps.
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Where to Get More Training
labsafety.tamu.edu/bloodborne.asp
Biological Safety
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Prevention of Exposures to Bloodborne Pathogens (BBP):
– Exposure to human blood and bodily fluids can result in
transmission of several bloodborne illnesses, including HIV and
Hepatitis B.
– This training presentation will aid in increasing awareness of the
risk of exposure to bloodborne pathogens and techniques and
practices for preventing exposure and abating hazards in
Engineering laboratories and facilities.
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Biological Safety
Prepared by:
http://engineering.tamu.edu/safety/
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Biological Safety
Biological Safety:
Safety for people, animals, plants
BioSafety and BioHazard Training
Office of Engineering Safety
Texas Engineering Experiment Station (TEES)
& The Dwight Look College of Engineering
“A safe, healthful, and secure environment for scholarship and research.”