Transcript COLIBACILLOSIS Cause : E. Coli

COLIBACILLOSIS
Cause : E. coli
‡ Especially attacked young
animals
‡ Human
:
» Infant
: Acute
gastroenteritis
» Adult : Cystitis
Animal
 Pig
- Gut Oedema : Odema Disease : BowI oedema
- Young pigs cause enteric colibacillosis
 Cow
- Secunder Infection Omphalitis, Arthritis, Cystitis,
Mastitis, Pyelonephritis
 Avian
- Hjarre’s Disease : Colligranuloma
- Omphalitis, Peritonitis, Salphingtis
ETHIOLOGY
‡ Fam. Enterobacteriaceae
‡ Live inside the GIT of mamalia - normal flora of
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intestine
Bad management and bad sanitasi
E. coli increase
E. coli : Bacillus coli : Bacterium Coli : Colon
Bacillus
Short stick (0,5 - 1,3 micron) gram (-)
Spora (-), capsule (-), motile
Aerob and facultative anaerob
 Easy grow at every media
 Ex : blood agar, Beta haemolitik
 Mac Conkey - wet red coloni
 EMBA - green methalic coloni with black at the
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center
Fluid media - muddy
Sensitive with dry conditing and desinfectan
Death at 600 c for 30 mnt
Inside ice cube still alive for 6 month
E. COLI GROOVE CAUSE OF ENTERISTIS
1.
2.
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4.
E. Coli Entero Toxigenic (ETEC)
E. Coli Entero Pathogenic (EPEC)
E. Coli Entero Invasive (EIEC)
E. Coli Adhesive and Emerge (AEEC)
EPIZOOTIOLOGI
» In Indonesia several cause
» Less information
» At a common disease for young
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animals
Young cow → † 25-30%
Young horse → † 25%
Young pig → † 50%
LOSS FINANCIAL
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Valve of treatment
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Body weight losses
PATHOGENESIS
MECHANISMS OF INFECTION
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Source of infection
- Feces
- Surrounded
- Salphingitas
- Matching egg
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Route of infection
- GIT
- Milk
- Intra uterine infection
Vicious disease depend on
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2.
Status imunity of the host
Bact. ability to produce toxin
 Cow
a. Septicaemia colibacillosis
b. Enteric toxaemia colibacillosis
c. Enteric Colibacillosis
 Pig : cause 3 types of enteric colibacillosis
a. Neonatal enteritis
b. Weanling enteritis
c. Edema Disease (enterotoxaemia)
 Sheep (2 type)
a. Enteric : proliferation groove
E. Coli → enterotoxin out come
b. Bacteremic
CLINICAL SYMPTOM
 Horse
a. Young horse
Symptom temp, pulse ↑, weakness + after 24 hour
b. ♀ Horse
Cause metritis after dystokia
Dog
» Fading puppy syndrom
» Symptom
weakness, anorexia, +
» Dog
» 70% cause of pyometra
CHICKEN
 Young chicken
3 weeks cause of pericarditis
 Chicken
resp. tract with IB virus and ND
BACTERY DIFFUSE IN BLOOD
Cause :
a. Acute colisepticaemia
b. Serositis
c. Coligranuloma (Hjarre’s Disease), with spesific tag
granulomatous at the intestine wall, liver and pulmo
Infected :
a. Inhalation
b. Egg contamination with faeces
YOUNG COW
3 types of colibacillosis
a. Enteric toxaemia colibaccillosis, collaps,
┼ in 2 - 6 hour, diarrheae (-)
b. Septicaemia colibacillosis, acute, death
for 1-4 days.
c. Enteric colibacillosis, attacks
1-3 weeks, fatty feces, ┼ 3-5 days
YOUNG SHEEP
type :
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Septicaemia per acute and enteric
chronic
Sensitive 1-2 days
Per acute, sudent death
Chronic, occur arthritis
YOUNG PIG
 Attack 1 - 3 days ages
 Sudden death for 24 hours without clinical
symtomp
 Enteric colibacillosis, attacked 8 - 16 weeks
 Clinical symtomp, depresion, anorexia,
40,50C, diarrhaea
PA CHANGES
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Enteric toxaemia and septicaemic
Colibacillosis non specific
Gastritis, enteritis
Avian : Pericarditis, peritonitis
Chicken : omphalitis
DIAGNOSIS
Material specimen
‡ Cardiac blood + coagulant
‡ Intestine + contents
‡ Mesenterials
‡ Lien
‡ Cerebrospinal fluid
‡ Faecal swab
Diagnosis based on
clinical diagnosa
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Difficult because of the manifestation
of the disease similar with other
disease
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Bacteria isolated and identification
Isolated
Identification: sugar, biochemisty
DIFFERENTIAL DIAGNOSA
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Salmonellosis
Diarrheae because of
feed
Acute septic of disease
DISEASE CONTROL
1.
Prevention
- Avoid from the the denity
- Diarrheae because of feed
- Acute septic of disease
2.
Administration
- The farmer should report rapidly
- D/by lab
3.
R/
- Oxytetracycline
- Streptomycine
- Kanamycin
SALMONELLOSIS
INTRODUCTION
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Infectious Disease in animals & human
Caused by Salmonella
Food Borne Disease (eggs, milk and meat
products)
Animals 
Decrease of production
Enteric Disease
Abortus and neonatal death
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Fowl 
High Mortality rate (DOC up to
2 – 3 weeks of age)
Etiology
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Salmonella  road shape cell, Gram negative, non
spore, somatic (O) & flagellar (H) antigens
Salmonella are usually motile except in poultry S.
gallinarum & S. pullorum, do not ferment lactose.
Growth optimal in MCA, SSA
The genus Salmonella contains more than 2.400
serotypes, occur worldwide and infect many
mammals, birds and reptiles and are mainly
excreted in faeces.
Epizootiology
Salmonellosis is a worldwide zoonosis, besides
economic losses to animal production, its zoonotic
character makes it an important public health
problem.
Organism may be present in water, soil, animal
feeds,raw meat and in vegetable material.
The source of environtmental contamination is
faeces
In poultry  S. pullorum infect the ovaries, and the
organisms can be isolated from eggs.
Pathogenesis
Although many aspects of the pathogenesis of Salmonellosis
are poorly understood, the virulence of Salmonellae relates to
their ability to invade host cells, replicate in them and resist both
digestion by phagocytes.
Peroral  tract. Intestinalis (mucosae of the illeum, caecum and
colon) and the messenteric lymph nodes  replicate and LPS
product endotoxin  it may be contribute to the local
inflammatory response which damages intestinal epithelial cells
 diarrhoea  organisms can be excreted through faeces
Bacterial cell wall LPS also mediates the endotoxic shock 
septicaemic salmonellosis
Latent infections, in which Salmonellae are present in the gall
bladder but are not excreted.
Clinical Symptom
Clinical disease may develop from subclinical and latent
infections if affected animals are stessed e.g
transportation, overcrowding etc.
Salmonellosis in adult cattle is usually sporadic and is also
often associated with stress.
In most animals species, both enteric and septicaemic forms
of salmonellosis are recorded.
•Enteric salmonellosis :
Acute disease is characterized by fever, depression,
anorexia and profuse diarrhoea often containing blood,
mucus and epithelial casts. Dehydration and weight loss
follow and pregnant animals may abort.
Chronic disease :
Can follow acute Salmonellosis in pigs, cattle and
horses are characterized by : intermittent fever,
soft faeces and gradual weight loss, emaciation.
•Septicaemic Salmonellosis :
Clinical disease is sudden with high fever,
depression, and die within 48 hours.
Surviving animals can develop persistent
diarrhoea, arthritis, meningitis or pneumonia.
Pigs  characteristic bluish discolouration of the
ears and snout.
Salmonellosis in poultry
S. pullorum  Pullorum disease
S. gallinarum  Fowl typhoid
S. enteritidis in egg dishes may result in human food poisoning
PULLORUM DISEASE = Bacillary White Diarrhoae 
Infects young chicks and turkey poults up to 2 – 3 weeks age and the
mortality rate is high
The clinical symptoms are anorexia, depression, whitish faecal pasting
around their vents and characteristic lessions include whitish nodes
throughout the lungs and focal necrosis of liver and spleen.
FOWL TYPHOID 
Lession in young chicks and poults similar of Pullorum Disease
Characteristic findings bile-stained liver and enlarge spleen.
Differentiation of the biotypes of S. pullorum and S.
gallinarum :
S. pullorum
S. gallinarum
Glukose (gas)
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Dulcitol
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Maltose
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Ornithine decarboxylase
+
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Rhamnose
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Motility
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Diagnosis
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A history of previous outbreaks of the disease, clinical sign and
postmortem picture.
Laboratory confirmation is required
Specimens : faeces, blood, intestinal contents, tissue lession
and abomasal contents ( aborted foetuses )
- Isolation and identification  specimen should be
cultured onto SSA, BGA and also added to Selenite F,
Tetrathionate broth for enrichment.
- Serrological tests  Rapid Whole Blood Test
(RWBT) for
Pullorum Test with Polyvalent antigen
or ELISA and DNA
probes.
Treatment
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Antibiotic therapy should be based
on results of susceptibility testing
Oral antimicrobial therapy should be
used judiciously for treating enteric
salmonellosis
Fluid and electrolyte replacement
therapy is required to counteract
dehydration and shock
Control
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Measures for excluding infection
form a herd or flock free of
salmonellosis
Measures for reducing
environmental contamination
Thank You