Transcript Prodigiosin Synthesis

Prodigiosin Synthesis
UV-induced “Colorless” Mutants of
Serratia marcescens
The Normal Pathway to Prodigiosin
alanine
amino
acids
proline
enzyme x
MAP(g)
Prodigiosin
enzyme z
enzyme y
O
MBC(ws)
N
H
N
H
N
Serratia marcescens D1 (wild type)
(DNA is not mutated for any of the genes)
ALA
amino
acids
PRO
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
Prodigiosin
Serratia marcescens D1 (wild type)
(DNA is not mutated for any of the genes)
ala
amino
acids
pro
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
Prodigiosin
Serratia marcescens D1 (wild type)
(DNA is not mutated for any of the genes)
ala
amino
acids
pro
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
Prodigiosin
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ala
amino
acids
pro
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
Prodigiosin
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
PRO
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
Prodigiosin
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
Prodigiosin
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
ala
amino
acids
pro
enzyme x
MAP(g)
enzyme z
enzyme y
enzyme x
MBC(ws)
MAP(g)
enzyme z
enzyme y
Prodigiosin
Prodigiosin
MBC(ws)
Serratia marcescens (
(DNA is mutated in enzyme y gene)
)
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
ALA
amino
acids
PRO
enzyme x
MAP(g)
enzyme z
enzyme y
enzyme x
MBC(ws)
MAP(g)
enzyme z
enzyme y
Prodigiosin
Prodigiosin
MBC(ws)
Serratia marcescens (
(DNA is mutated in enzyme y gene)
)
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
ala
amino
acids
PRO
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
enzyme x
MAP(g)
enzyme z
enzyme y
Prodigiosin
Prodigiosin
MBC(ws)
Serratia marcescens (
(DNA is mutated in enzyme y gene)
)
Serratia marcescens (
)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
ala
amino
acids
PRO
enzyme x
MAP(g)
enzyme z
enzyme y
MBC(ws)
enzyme x
MAP(g)
933
enzyme z
enzyme y
Prodigiosin
WCF
Prodigiosin
MBC(ws)
Serratia marcescens (
(DNA is mutated in enzyme y gene)
)
Serratia marcescens (933)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
ala
amino
acids
PRO
enzyme x
MAP
MAP(g)(g)
enzyme z
enzyme y
MBC(ws)
enzyme x
MAP(g)
Prodigiosin
Prodigiosin
933
MAP(g)
enzyme z
enzyme y
WCF
Prodigiosin
MBC(ws)
Serratia marcescens (WCF)
(DNA is mutated in enzyme y gene)
Serratia marcescens (933)
(DNA is mutated in enzyme x gene)
ALA
amino
acids
pro
enzyme x
MAP(g)
Prodigiosin
enzyme z
enzyme y
MBC(ws)
933
WCF
MBC(ws)
ala
amino
acids
PRO
enzyme x
MAP(g)
enzyme z
enzyme y
Prodigiosin
Prodigiosin
MBC(ws)(ws)
MBC
Serratia marcescens (WCF)
(DNA is mutated in enzyme y gene)
Serratia marcescens D1 (wild type)
(DNA is not mutated for any of the genes)
ala
MAP(g)
enzyme x
amino
Prodigiosin
enzyme z
acids
MBC(ws)
pro
enzyme y
Why is D1 (so capable!) such a poor donor to either mutant…
D1
933
D1
WCF
MAP(g)
MBC(ws)
933
WCF
MAP(g)
MBC(ws)
…compared to cross-feeding from either mutant?