Transcript New Graduate Student Orientation Power-Point

ANIMAL WELFARE TRAINING - 2013
ANIMALS in RESEARCH & TEACHING
BIOMEDICAL MODELS & BASIC STUDIES
W.L. Shelton, Director
Laboratory Animal Resources
Institutional Animal Care and Use Committee
University of Oklahoma
http://iacuc.ou.edu
Time Line: 1966 - Public Law promulgation = Animal Welfare Act
1985 – USDA & NIH regulation revisions – established IACUC requirement
UNIVERSITY OF OKLAHOMA
STRUCTURE of ANIMAL CARE
and USE PROGRAM*
LABORATORY ANIMAL RESOURCES (LAR)
INSTITUTIONAL ANIMAL CARE AND USE
COMMITTEE (IACUC)
*Mandated by AWA of 1966 and amendments
Animal Care & Use Program
Implies the two major programmatic functions
—
CARE = Animal husbandry = LAR
USE = Experimental Aspect = IACUC
STUDY and TEACHING CATEGORIES*
Laboratory vs. Field – Manipulate vs. Observation
Basic Studies (biology, behavior, husbandry)
Natural History Studies – native species in natural
habitat
Animal Models (genetics, physiology, medical,
human surrogate) – Lab mammals, fish, amphib.
Biomedical –Lab Animals or Farm Animals or Native
species
Agricultural – Husbandry (Simulate farm environ.)
-
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*ALL LIVE VERTEBRATES COVERED BY ANIMAL
WELFARE ACT
ETHICAL ISSUES
HUMAN SUBJECT - Testing/Experimentation?
- Institutional Review Board - Required
- Permission - Required
- Placebos?
- Drugs/Procedures?
ANIMALS – Cannot Obtain Permission!! Therefore:
- Well being – Animal welfare
- Appropriate animal model?
- Information Value – Justification
- Numbers – statistical validity/ minimum required
- Non-human Vertebrates – Sentience Consideration
- Pain/ Stress alleviation – Required & Explained
Not everyone is enthusiastic about
using animals in research
IS IT NECESSARY / IMPORTANT?
USE of ANIMALS in RESEARCH
and TEACHING
Opponents vs Proponents
Animal Rights vs Animal Welfare
Animal Protection Societies were instrumental in the stimulation of
legislation and subsequent development of the mechanism for
animal welfare protection relative to biomedical research
ANIMAL
WELFARE
PERSPECTIVE
Ecoterrorist and Protest groups have developed and
operate outside the law
ANIMAL RIGHTS PERSPECTIVE
PETA opposes animal
use – entertainment,
food, experimentation.
Not above radicalization
ANTHROPOMORPHISM IS NOT
SCIENTIFIC
STRUCTURE of ANIMAL CARE
and USE PROGRAM*
LABORATORY ANIMAL RESOURCES
(LAR)
INSTITUTIONAL ANIMAL CARE and USE
COMMITTEE
(IACUC)
*Mandated by AWA of 1966 and amendments
THE UNIVERSITY OF OKLAHOMA
(A3240-01)
ANIMAL WELFARE ASSURANCE
in accordance with the PHS Policy for
Humane Care and Use of Laboratory Animals
Update Approved 10 June 2013
In effect through 30 June 2017
MANUAL of STANDARD OPERATING PROCEDURES (M-SOP)
LABORATORY ANIMAL RESOURCES (LAR)
UNIVERSITY OF OKLAHOMA, NORMAN CAMPUS (OUNC)
February 2012 (3rd Edition)
January 2009 (2nd Edition)
May 2005 (1st Edition)
William L. Shelton,
LAR Director
Sutton Hall, 306
405-325-1058
[email protected]
Administrative Office
Sutton Hall, 308c
405-325-2609
Mailing address
730 Van Vleet Oval
Richards Hall, 314
University of Oklahoma
Norman, OK 73019
ANIMAL ORDER APPROVAL & CONFIRMATION FORM
LABORATORY ANIMAL RESOURCES
Complete this form and send it to Director, Laboratory Animal Resource, Department of
Biology, Richards Hall, Room 314.
Project Name & Animal Use Statement Number (R0X-XXX)
Project Number or Course Number: ____________________
Principal Investigator/ Instructor: ______ Phone #:_______
Housing of Animals: Building: _______ Room#: _______
Single : ____Group:______ Number Per Cage:____
NOTE: Animals will be segregated by sex unless otherwise directed.
Ordering Information:
Vendor: ______________________ Phone # Number:____
Name of Person Placing the Order: _______________
Species: ______ Sex: ________ Strain/Stock______________
Weight/Age: ________
Date Ordered: _________ Expected Deliver Date: _________
Deliver Animals to: ________________________________
Special Requirements:
Received in LAR office:________________ by:___________
Processed Form will be sent to the P.I./Instructor, the Animal Facilities Manager, and to the
appropriate stockroom manager if ordered on P-card.
Update July 2013
University of Oklahoma, Norman Campus
Animal Procurement, Use, and Disposal Procedures1
All Investigators must follow the appropriate procedures of the
University of Oklahoma, Norman Campus LAR for procurement, use,
and disposal of laboratory animals.
a.
b.
c.
d.
Ordering
Complete the animal order form and email to LAR Director.
Receive confirmation to proceed with the order; a processed copy of the
order will be sent to the PI, animal facilities manager and to the
appropriate Stockroom manager for P-card purchases.
Call in order as described.
Be available to receive the animals at delivery location where the animals
will be housed.
Receiving
a. The PI receives the animals and places in the appropriate cages which
have been prepared by LAR personnel.
b. Investigators will be responsible for the welfare of the animals until they
are delivered to the housing facilities.
Housing
a. Housing location will be determined by the Office of Lab Animal
Resources, with the Investigator’s concurrence.
b. Location of the research and well-being of the animals will be primary
considerations.
Disposal
a. Biology PI’s & instructors will deliver carcasses and waste material to
the stockroom in Richards Hall, others will deliver this material to the
Animal House. A special pickup each semester may be arranged for
material from Comparative Anatomy.
b. Carcasses will be placed in plastic bags; no laboratory or surgical trash
should be included.
c. Identify contents on a label, include investigator’s name, and whether
contents have biohazards, infectious, or radioactive materials.
STRUCTURE of ANIMAL CARE
and USE PROGRAM*
LABORATORY ANIMAL RESOURCES
(LAR)
INSTITUTIONAL ANIMAL CARE and USE
COMMITTEE
(IACUC)
*Mandated by AWA of 1966 and amendments
IACUC
• Self-regulatory entity with responsibility to
monitor animal research and the animal
care program at institutions that use
Live Vertebrate Animals
IN RESEARCH or TEACHING
APHIS/USDA – Registered Research Facility
OLAW/PHS - Institutional Assurance
Compliance Document
= 4-year ‘contract’
Training
• Federal law requires training for all who use
live animals in research & teaching
• Type of training depends on role
– Supervisor and husbandry staff
– Veterinarians
– IACUC Members
– Investigators
Signature of Major Professor -- Letter
Faculty and/or Graduate Student
Research involving live
vertebrate animals
--IACUC approval must precede research
--Documentation of IACUC review is
required by funding agencies & University:
Office of Research Support – proposals
Graduate College – thesis check-off
--Students must be named in approval
letter if working on your Major Prof’s.
AUS, OR You must have your own!
IACUC Review Verification
for thesis/dissertation research
M.S. – COMMITTEE MEETING
THESIS TOPIC FORM – TOO LATE!
DOCTORAL – ADVISORY CONFERENCE
OK
iacuc.ou.edu
Application procedure & forms
Links and Announcements
Training
IACUC Responsibilities*
•
•
•
•
Review Program – Semi-annually
Inspect Laboratory & Housing facilities – d.o.
Investigate suspected violations of AWA
Research Protocol Review - Monthly
Personnel qualifications (Requisite training)
Description of Literature Search
Justification – protocol, species, numbers,
& objectives
Procedures to mitigate pain/distress
Appropriate analgesia/anesthesia/euthanasia
__________________________
*Report to Institutional Official
ELECTRONIC LITERATURE
SEARCH
• Background or Foundational Studies
• Non-duplication
• Appropriate procedures
• Correct Animal Model
*Sentience? Pain?
*(Conscious awareness of stimuli)
Cerebral Cortex in CNS
• Reactivity can & does occur without sentience
• Many Complex Behaviors can be done without
conscious awareness, including learned avoidance
• Pain is sensed by cerebral cortex where it is perceived
and processed, but with no definite physical dimension.
• However – USDA definition of Pain relative to AWA
Evolution of the Vertebrate CNS
Evolutionary Adaptation
CNS – Physiology - Behavior
RESEARCH & TEACHING
Biomedical
Ecological
Agricultural
iacuc.ou.edu
Thank You – Questions?