Transcript yoshizaki_june4_2011

Historical Perspectives: Role of Creatine
Phosphate in Energy Transport in Muscle
Cells Evaluated by 31P-NMR.
Kazuo Yoshizaki
Department of Physiology, School of
Medicine, University of Tokushima,
Tokushima 770-8503, JAPAN
[email protected]
Ｐ／Ｏ
＝
２
～
３
Shuttle hypothesis
a) ATP carrier model
energy-production system
(mitochondria)
ATP
ADP
energy
energy-consumption
system
(actomyosin)
ATP
ADP
energy
+
Pi
Pi
ｂ) C~P carrier model
energy-production system
ATP
energy
ADP
C
C~P
Pi
energy-consumption
system
C
C~P
Pi
ATP
ADP
energy
Intracellular Energy Transport in Muscle:
Yoshizaki et al BBA 1051:144 (1990)
1) Diffusivities of phosphorus metabolites
measured by 31P-pulsed gradient NMR.
2) The flux of creatine kinase reaction
measured by 31P-saturation transfer NMR.
3) Evaluation on creatine phosphate shuttle
theory.
Dr. G.K. Radda’s group at Oxford, U.K., 1983.
Diffusion distance
2Dτ
τ: life time
D: diffusion coefficient
ATP
22 μm
ADP
1.8
Phosphocreatine
57
Creatine
37
0
10
20
30
40
50
Diffusion distance (μm)
60
70
2Dτ
A case report on acute myocardial infarction accompanied
with no increase in serum creatine kinase activity.
K. Koizumi et al., Kobe City General Hospital,
J. Jap. Coll. Angiol., 27, 988 (1987) (in Japanese)
A 56 years old female, suffering from Diabetes mellitus without
cardiac complications, died with acute myocardiac infraction.
The agar gel electrophoresis of muscle homogenates showed that
the CK-MM isozyme was almost undetected in skeletal and cardiac muscles,
and the CK-MB isozyme was decreased in cardiac muscle.
(Tentatively translated by K. Yoshizaki)
P 2-4 血清ＣＫ上昇を伴わない急性心筋梗塞症の一例
神戸市立中央市民病院 循環器センター
同臨床病理科*
小泉克己， 吉川純一， 加藤 洋， 奥町冨久丸，
吉田 清， 白鳥健一， 高尾精一， 赤阪隆史，
赤土正洋， 城 泰子， 笠倉新平*， 内田博也*
Shuttle hypothesis
a) ATP carrier model
energy-production system
(mitochondria)
ATP
ADP
energy
energy-consumption
system
(actomyosin)
ATP
ADP
energy
+
Pi
Pi
ｂ) C~P carrier model
energy-production system
ATP
energy
ADP
C
C~P
Pi
energy-consumption
system
C
C~P
Pi
ATP
ADP
energy
Biochmica et Biophysica Acta, 1074 (1991) 302-311
In vivo regulation of mitochondrial respiration in cardiomyocytes:
Specific restrictions for intracellular diffusion of ADP
Valdur A. Sakes, Yulia O. Belikova and Andrey V. Kuznetsov
Laboratory of Bioenergetics, U. S. S. R. Cardiology Research Center, Moscow (U. S. S. R.)
Preparations
Km(μM)
Km(mM)
ADP
no additions
Mitochondria
+AP5A
17.6± 1.0
+(creatine)
13.6±4.4
creatine
(at 0.2mM ATP)
6.9 ±0.14
Saponin-treated
carduinyocytes
Skinned fibers
250.2±38.5
263.7±57.4
256.5±31.8
35.6±5.6
79
±8
5.67±0.11
6.25±0.21
Biochmica et Biophysica Acta, 1074 (1991) 302-311
In vivo regulation of mitochondrial respiration in cardiomyocytes:
Specific restrictions for intracellular diffusion of ADP
Valdur A. Sakes, Yulia O. Belikova and Andrey V. Kuznetsov
Laboratory of Bioenergetics, U. S. S. R. Cardiology Research Center, Moscow (U. S. S. R.)
Discussion:
(The old Japanese lady, described
by
Preparations
K (μM)
K (mM)
ADP reported to have only 2% creatine
Oita et al. (1988)
of
intact
(at 0.2mM ATP)
no additions
+AP A
+(creatine)
creatine kinase preserved, might not be able to
perform
of work any more
and might
had
Mitochondriatoo much
17.6± 1.0
13.6±4.4
6.9 ±0.14
Saponin-treated
suffered
from decreased relaxation; however, such a
carduinyocytes
250.2±38.5
256.5±31.8
35.6±5.6
5.67±0.11
case
should
be263.7±57.4
verified by more 79numerous
studies
Skinned
fibers
±8
6.25±0.21 to
be taken seriously.)
m
m
5
31P-saturation
transfer NMR on skeletal muscles
of creatine kinase (CK)-knockout mice.
wild
M-CK(-/-)
Nicolay et al. (1998)
Mol. Cell. Biochem 184: 195-208
31P-saturation
transfer NMR on skeletal muscles
of creatine kinase (CK)-knockout mice.
wild
M-CK(-/-)
Nicolay et al. (1998)
Mol. Cell. Biochem 184: 195-208
1983