Transcript Starbind

STARBIND I In vitro
nEvaluating binders in vitro: example
Solution of toxins
10 µg/l Afl + 2 g/l binder
(pH-traject)

Afltotal: 10 µg/l
Aflfree: 2 µg/l
 Aflbound: 8 µg/l Afl
 adsorption: 80%

equilibrium
Measure conc. free
toxin( ) at equilibrium
in solution
 2 µg/l Aflfree
Toxin + binder  complex
Starbind | In vitro
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nClay minerals - 2003
Starbind
Clinoptilolite Clay minerals
Zearalenone
Clay minerals
2000 mg/metric ton feed
5 kg/metric ton feed
pH 2.5
Yeast product
pH 8.0
Organic acids
and salts
Adsorption of 1040 mg ZEA/MT feed
=52%
Starbind | In vitro
Slide 3
nClay minerals & yeast - 2004
Starbind
Clinoptilolite Clay minerals
Aflatoxin
340 mg/metric ton feed
Ochratoxin A
1000 mg/metric ton feed
Clay min.& yeast 5 kg/metric ton feed
pH 3.0
Yeast product
pH 6.5
Organic acids
and salts
Adsorption of
230 mg aflatoxin/MT feed = 67%
302 mg ochratoxin A/MT feed = 30%
Starbind | In vitro
Slide 4
nClinoptilolite - 2006
Starbind
Clinoptilolite Clay minerals
Aflatoxin
Zearalenone
Clinoptilolite
400 mg/metric ton feed
2000 mg/metric ton feed
10 kg/metric ton feed
pH 3.0
Yeast product
pH 6.5
Organic acids
and salts
Adsorption of
398 mg aflatoxin/MT feed = 99%
250 mg zearalenone/MT feed = 12.5%
Starbind | In vitro
Slide 5
nStarbind in commercial feed
Aflatoxine
adsorption [%]
contamination [ppb]
100
90
80
70
60
50
40
30
20
10
0
60
50
40
30
[ppb]
[%]
Clinoptilolite Clay minerals
Starbind
20
10
0
Breeder feed Breeder feed Breeder feed
80023
80026
80027
Feed 213/1
Feed 213/2
pH 6.5
(3h)
Fumonisine
contamination [ppm]
100
90
80
70
60
50
40
30
20
10
0
Adsorption of
Aflatoxine
ca 95%
Fumonisine. ca 63%.
8
7
6
5
4
3
[ppm]
[%]
Yeast product
adsorption [%]
Organic acids
and salts
Starbind
1kg/MT feed
Aflatoxine
diff. contamin
Fumonisine. diff. contamin.
2
1
0
Breeder feed Breeder feed
80023
80026
Breeder feed
80027
Feed 213/1
Feed 213/2
Kasetsat University Thailand, 2008
Starbind | In vitro
Slide 6
nScreening project 2009
Product
Mycotoxin
pH
Dosage
Starbind
ZEA, OTA, AFL,
at 5, 15, 50,
150 and 500
ppb
3 & 6,5
2 kg / Ton
Binder F
Binder M
Starbind | In vitro
Slide 7
nScreening project 2009 - ZEA
100
Percentage bound
80
60
Free-Tox
Binder F
Binder M
40
20
0
5
15
50
150
500
contamination (µg/L)
Starbind | In vitro
Slide 8
nScreening project 2009 - OTA
100
Percentage bound
80
60
Free-Tox
Binder F
Binder M
40
20
0
5
15
50
150
500
contamination (µg/L)
Starbind | In vitro
Slide 9
nScreening project 2009 - AFL
100
Percentage bound
80
60
Free-Tox
Binder F
Binder M
40
20
0
5
15
50
150
500
contamination (µg/L)
Starbind | In vitro
Slide 10
Starbind I In vitro – adsorption isotherm
nAdsorption isotherm: example
Solution of toxins
10 µg/l AF + 2 g/l binder
(pH-traject)

AFtotal: 10 µg/l
AFfree: 2 µg/l
 AFbound: 8 µg/l AF

equilibrium
Measurement of free
toxin( ) at equilibrium
In solution
 2 µg/l AFfree
Toxin + binder  complex
 Per gram binder: 4 µg AF adsorption
 Repeatment with the same dosage of toxin binder at higher concentration of toxin
Starbind | In vitro
Slide 12
nInterpretation of adsorption-isotherm
28
33%
12%
12%
9%
44%
20
80%
16
24%
32%
12
70%
80%
8
90% 80%
4
90%
200
190
180
170
160
150
140
130
120
110
100
90
80
70
60
50
40
30
20
10
0
0
Bound toxin [µg/g binder]
24
16%
Contamination [µg/kg]
The steeper the slope  the bigger the affinity to the toxin
The higher the saturation line  the higher the max. binding capacity
Starbind | In vitro
Slide 13
nIsotherm voor Starbind:
Starbind
Yeast product Clinoptilolite Clay minerals
Zearalenone
Starbind
Binder G
0-5-10-50-150-500 ppb
2 kg/MT feed
2 kg/MT feed
pH 3.0 (1h) pH 3.0 (1h)
pH 6.5 (3h)
Organic acids
and salts
Isotherm
Starbind | In vitro
Slide 14
nZEA binding
Adsorption
isotherm:
Binder Starbind
Binder G
bound zearalenone [ppb]
300
250
200
150
100
50
0
0
Conclusion:
50
100
150
200
250
300
350
400
450
500
free zearalenone [ppb]
 The Starbind isotherm shows a steeper slope  higher affinity to ZEA
 Binder G shows a saturating slope
Starbind | In vitro
Slide 15
nZEA binding
Binder Starbind
Binder G
Free zearalenone [ppb]
500
450
400
350
300
250
200
150
100
50
0
0
50
100
150
200
250
300
350
400
450
500
550
contamination [ppb]
Conclusion:
Starbind can bind more ZEA
Starbind can reduce a higher contamination (120 ppb) to a safe level than binder G
Starbind | In vitro
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