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Electronic Supplementary Material (ESI) for Nanoscale.
This journal is © The Royal Society of Chemistry 2017
ElectronicSupplementaryInformation(ESI)
Citrate-stabilizedGoldNanoparticleshinderfibrillogenesisofapathologic
variantofβ2-microglobulin
a
b,c
d
a,c
b,c
CristinaCantarutti, SaraRaimondi, GiorgiaBrancolini, AlessandraCorazza, SofiaGiorgetti, Maurizio
e
e
f
g
b,c
Ballico, StefanoZanini, GiovanniPalmisano, PaoloBertoncin, LoredanaMarchese, P.Patrizia
b,c,h
b,c,h
d
a,c
a,c,e
Mangione, VittorioBellotti, StefanoCorni, FedericoFogolari, andGennaroEsposito
a
DSMB,UniversitàdiUdine,P.leKolbe4,33100Udine,Italy,
DipartimentoMedicinaMolecolare,UniversitàdiPavia,ViaTaramelli3,27100Pavia,Italy,
INBB,VialeMedaglied’Oro305,00136Roma,Italy,
d
CNRIstitutoNanoscienze,ViaCampi213/A,41125Modena,Italy,
e
ScienceandMathDivision,NewYorkUniversityatAbuDhabi,AbuDhabi,UAE,
f
DepartmentofChemicalandEnvironmentalEngineering,MasdarInstituteofScienceandTechnology,POBox54224,AbuDhabi,UAE,
g
DipartimentoScienzedellaVita,UniversitàdiTrieste,ViaWeiss2,34128Trieste,Italy,
h
DivisionofMedicine,UniversityCollegeofLondon,LondonNW32PF,UK.
b
c
S1
Fig. S1 Bar plots of individual peak attenuations calculated as intensity ratios of the
corresponding signals in the HSQC spectra of D76N β2m with and without Cit-AuNPs at
protein/NP ratio of 53 (a), 107 (b) and 213 (c). The two horizontal lines indicate the
standarddeviationlevelsaboveandbelowtheaverageattenuation.
S2
Fig.S2PrincipalComponentanalysis:firstthreedominantfluctuationsofthe(a)firstD76N
β2m monomer in the dimer and (b) second D76N β2m in the same dimer simulated in
solvent.ThefirstproteinhasdistortionswhichcorrelateABandDEloopmotionswhichare
notablylargerthanthoseofthesecondprotein.
S3
Fig. S3 Native agarose gel electrophoresis of 20 µM D76N β2m in non-fibrillogenic
conditions(a)withoutCit-AuNPsand(b)withCit-AuNPs,atdifferentincubationtimes.The
lossofproteininpresenceofCit-AuNPscanbeobservedalsoinnon-fibrillogenicconditions:
theamountoffreeproteinislowerifitisincubatedwithCit-AuNPs(b)withrespecttothe
control (a). In addition, the second species detected in presence of Cit-AuNPs when the
proteinisexposedtofibrillogenicconditions(seeFig.10binthemaintext)isabsent.
S4
Fig.S4Nativeagarosegelelectrophoresisof20µMD76Nβ2mincubatedunderfibrillogenic
conditions(a)withoutCit-AuNPsand(b)withCit-AuNPs,atdifferentincubationtimes(the
blackboxindicatestheunknownspeciesthatwasinvariablyobservedinthepresenceofCitAuNPs). In (c) only Cit-AuNPs were loaded on the gel as a control of the absence of any
stainingduetoCit-AuNPs.
S5
Fig.S5Nativeagarosegelelectrophoresisof20µMD76Nβ2mincubatedunderfibrillogenic
conditions:(a)and(c)withoutCit-AuNPsand(b)and(d)withCit-AuNPs.Thegelsreported
in (c) and (d) were run after long centrifugation (14000 rpm, 30 minutes) that ensures
completeprecipitationofCit-AuNPs,ascheckedbyUV-Vis.Evenin(d)thepresenceofthe
unknownspecies(boxedinblack)isnoticeable,confirmingtheabsenceofnanoparticlesin
thisspecies.
S6