Transcript Yeast Lab Instructions

YEAST LAB
DEAD YEAST W/MINERAL OIL
• 2 mL of sucrose solution to 50 mL beaker
• Add 2 mL of DEAD yeast; pipette up and down 2-3x to
mix
• Cover this solution with mineral oil
• Incubate beaker in warm water bath (38°-42°C) for 5
min
• Place both beakers into biochamber, insert probe and
press collect (data should collect every 15 seconds for 4
minutes)
• After data collection, restore warm water bath
temperature
• Fan air over bio-chamber to restore gas levels
YEAST LAB
DEAD YEAST W/O MINERAL OIL
• Pipette the yeast solution from under mineral oil
to clean 50 mL beaker (better to transfer less
yeast than to transfer mineral oil)
• Incubate beaker in warm water bath (38°-42°C)
for 5 min
• Place both beakers into biochamber, insert probe
and press collect
• When asked, choose ‘store latest run’
• Data should collect every 15 seconds for 4
minutes
YEAST LAB
LIVE YEAST W/MINERAL OIL
• 2 mL of sucrose solution to 50 mL beaker
• Add 2 mL of LIVE yeast; pipette up and down 2-3x to
mix
*Be careful, pipetting too vigorously will kill the
yeast
• Cover this solution with mineral oil
• Incubate beaker in warm water bath (38°-42°C) for 5
min
• Place both beakers into biochamber, insert probe and
press collect, click ‘store latest run’ (data should collect
every 15 seconds for 4 minutes)
• After data collection, restore warm water bath
temperature
• Fan air over bio-chamber to restore gas levels
YEAST LAB
LIVE YEAST W/O MINERAL OIL
• Pipette the yeast solution from under mineral
oil to clean 50 mL beaker (better to transfer
less yeast than to transfer mineral oil).
• Incubate beaker in warm water bath (38°42°C) for 5 min
• Place both beakers into biochamber, insert
probe and press collect (data should collect
every 15 seconds for 4 minutes)
• When asked, choose ‘store latest run’