Transcript slides - University of Verona

Fig. 1B
Fig. 1A
Th1 cells
Th2 cells
beta1
beta1
alpha4
alpha4
beta7
beta7
IL-4
Th1 cells
IFN-g
IL-4
Th2 cells
IFN-g
Mean fluorescence Intensity (log scale)
Fig. 1C
60
50
60
Th1 cells
50
40
40
30
30
20
20
10
10
0
0
300
700
1500
60
50
Th1 cells
1200
2.500
5000
Sticking
60
Th2 cells
50
40
40
30
30
20
20
10
10
Th2 cells
0
0
300
700
1500
MAdCAM-1 (sites/mm2)
Rolling
1200
2500
VCAM-1 sites/mm2)
5000
Th1 cells
Th2 cells
CCR4
CCR4
CXCR3
CXCR3
Fig. 2B
Chemotaxis (arbitrary units)
Fig. 2A
2500.0
MDC/CCL22
Th1
Th2
2000.0
1500.0
1000.0
500.0
0.0
1
1600.0
1400.0
1200.0
1000.0
800.0
600.0
400.0
200.0
0.0
10
1000
I-TAC/CXCL11
Th1
Th2
1
Mean fluorescence Intensity (log scale)
100
10
100
1000
Chemokine concentration (ng/ml)
Chemotaxis (arbitrary units)
Fig. 2C
1800.0
1600.0
1400.0
1200.0
1000.0
800.0
600.0
400.0
200.0
0.0
0.1
MDC/CCL22
TARC/CCL17
1
10
1800.0
1600.0
1400.0
1200.0
1000.0
800.0
600.0
400.0
Th2 cells
200.0
0.0
100
1000
1
Mig/CXCL9
I-TAC/CXCL11
Th1 cells
10
Chemokine concentration (ng/ml)
100
1000
Fig. 2D
Th1 cells
Th1 cells
Intracellular Calcium Mobilization (MFI)
CXCL9
0
CXCL11
100
200
300
400
CXCL11
500
0
CXCL9
100
Time (s)
CCL22
CCL22
200
300
400
500
0
100
200
600
Time (s)
300
400
500
800
1000
L1.2-hCCR4 cells
CCL22
400
200
Time (s)
L1.2-hCCR4 cells
0
500
CCL17
Time (s)
CCL17
400
Th2cells
cells
Th2
CCL17
100
300
Time (s)
Th2 cells
0
200
CCL22
800
1000
0
200
CCL17
400
600
Time (s)
CCR4= 8.4
TH1
CXCR4=20
beta1=144
alfa4=182
beta7=165
CXCR3=54
TH2
CCR4= 43.9
beta1= 270
CXCR4= 33.9
alfa4= 277
beta7= 217
CXCR3= 8
Dynamic cellular interactions under flow conditions
Glass capillary
2 dynes/cm2
Chemokine
Adhesive substratum
Interactions of Th1 and Th2 cells with MadCAM-1 under flow
Th1 cells
60
Number of cells in 0.2 mm2/30s
50
40
30
Total interactions
Rolling
Sticking >10s
20
10
0
0.1 mg/ml
0.5 mg/ml
1 mg/ml
Th2 cells
70
60
Total interactions
50
Rolling
40
Sticking >10s
30
20
10
0
0.1 mg/ml
0.5 mg/ml
MAdCAM-1
1 mg/ml
Interactions of Th1 and Th2 cells with MadCAM-1 under flow
Th1 cells
50
None
Number of cells in 0.2 mm2/30s
40
MDC
30
TARC
20
10
0
Total
interactions
Rolling
Sticking
>10 s
Th2 cells
50
None
40
MDC
30
TARC
20
10
0
Total
interactions
Rolling
MAdCAM-1
Sticking
>10 s
Interactions of Th1 and Th2 cells with MadCAM-1 under flow
Th1 cells
Th1 cells
120
100
None
100
80
IP-10
80
60
40
20
0
Total
interactions
Rolling
Sticking
>30 s
Th2 cells
120
100
None
80
IP-10
60
40
20
Number of cells in 0.2 mm2/30s
Number of cells in 0.2 mm2/30s
120
60
40
20
0
Total
interactions
Rolling
MAdCAM-1
Sticking
>30 s
Total
interactions
Rolling
Sticking
>30 s
Th2 cells
120
None
SDF-1
100
80
60
40
20
0
0
None
SDF-1
Total
interactions
Rolling
MAdCAM-1
Sticking
>30 s
Interactions of Th1 and Th2 cells with VCAM-1 under flow
Th1 cells
60
None
MDC
TARC
50
Number of cells in 0.2 mm2/30s
40
30
20
10
0
Total
interactions
Rolling
Sticking
> 10s
Th2 cells
60
None
MDC
TARC
50
40
30
20
10
0
Total
interactions
Rolling
VCAM-1
Sticking
> 10s
Interactions of Th1 and Th2 cells with VCAM-1 under flow
Th1 cells
120
100
None
80
IP-10
Number of cells in 0.2 mm2/30s
60
40
20
0
Total
interactions
Rolling
Sticking
> 10s
Th2 cells
120
100
None
80
IP-10
60
40
20
0
Total
interactions
Rolling
VCAM-1
Sticking
> 10s